Conjugation

Part:BBa_K873003:Design

Designed by: yue hu   Group: iGEM12_SEU_A   (2012-09-19)


conjugative transfer of RFG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 117
    Illegal NgoMIV site found at 127
    Illegal AgeI site found at 1014
    Illegal AgeI site found at 1126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 19


Design Notes

This part can just be moblized by other plasmids which contain tra gene.


Source

Both the two parts we used were from registry.

References